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m13 forward primer  (Thermo Fisher)


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Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
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    Structured Review

    Thermo Fisher m13 forward primer
    M13 Forward Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m13+forward+primers/m13+forward+primers/pm40508156-194-7-14
    Average 90 stars, based on 1 article reviews
    m13 forward primer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression.
    Article Snippet: The recombinant plasmids were sequenced in both directions using M13 forward and reverse primers (Invitrogen) and an ABI BigDye Terminator Cycle Sequencing Kit v3.1 (Thermo Fisher, Waltham, MA, USA) with a Gene Amp 9700 PCR System (Thermo Fisher, Carlsbad, CA, USA).

    Article Title: Organoids and metastatic orthotopic mouse model for mismatch repair-deficient colorectal cancer.
    Article Snippet: The purified amplicon then proceeded into cycle sequencing with BigDyeTM Terminator v3.1 Cycle Sequencing Kit and M13 forward and M13 reverse primers (Thermo Fisher Scientific) using the following conditions in the ProFlexTM PCR System: 96° C for 1 minute, 25 cycles of 96°C for 10 seconds, 50°C for 5 seconds, 60°C for 1 minute and 15 seconds; followed by a hold at 4°C.

    Article Title: Effects of hypertonic stress on transforming growth factor-beta activity in normal rat kidney cells.
    Article Snippet: Sequencing of the double stranded DNA was done using fluorescence labeled M13 forward or M13 reverse primers on an automated sequencer (Applied Biosystems Inc., Foster City, CA, USA) to confirm the sequences of the PCR products.

    Article Title: The Prognostic and Predictive Role of Somatic BRCA Mutations in Ovarian Cancer: Results from a Multicenter Cohort Study
    Article Snippet: Sanger sequencing was performed with predesigned Invitrogen M13 forward and reverse primers and the BigDyeTM Direct Cycle Sequencing Kit, sequenced with the Applied Biosystems® 3500xL Dx Genetic Analyzer platform and analyzed with Minor Variant Finder or SeqScape3 software (ThermoFisher Scientific).

    Article Title: Two Novel Bacterial Biosensors for Detection of Nitrate Availability in the Rhizosphere
    Article Snippet: The PCR products were confirmed by sequencing using M13 forward ( 20) and M13 reverse primers (Invitrogen, Carlsbad, CA) on an ABI model 377 DNA sequencer (ABI Prism).

    Article Title: Early Cell Cultures from Prostate Cancer Tissue Express Tissue Specific Epithelial and Cancer Markers
    Article Snippet: The recombinant plasmids were isolated by the DM miniprep method [ ] and sequenced in both directions using M13 forward and reverse primers (Invitrogen) and ABI BigDye Terminator Cycle Sequencing Kit v3.1 (Thermo Fisher, Waltham, MA, USA) with a Gene Amp 9700 PCR System (Thermo Fisher, Carlsbad, CA, USA).

    Clone Assay:

    Article Title: Cloning of a new form of EAAT2/GLT-1 from human and rodent brains.
    Article Snippet: Glutamate transporter 1 is the principal transporter that mediates glutamate clearance in the mammalian brain.. In rodents, it is referred to as GLT-1, whereas in humans it is referred to as EAAT2.. We have cloned a novel and abundantly expressed carboxyl-terminal splice variant of this transporter in both rodents and humans, which we denote as GLT-1d/EAAT2d.

    Sequencing:

    Article Title: Cloning of an outer membrane protein gene from Campylobacter jejuni.
    Article Snippet: An antigen in the outer membrane protein (OMP) fraction of Campylobacter jejuni was identified and characterized.. Western blot analysis demonstrated antigenic differences in this protein between two congenic C. jejuni strains.. Strain A74/C, which colonizes chickens, expressed the antigen at 34 kDa, while strain A74/O, which poorly colonizes chickens, expressed the antigen at 32 and 34 kDa.



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